· Yeast Transformation (Gietz Lab)
LiAc/SS-DNA/PEG Transformation
· Yeast Transformation (Breeden Lab)
LiAc method
· Large-Scale Yeast Transformation (Yale Yeast Analysis Center)
Detailed protocol for large-scale yeast transformation including competent cell preparation, transfromation...
· Yeast Transformation (Herskowitz Lab)
· Sleazy Plate Transformation of Yeast (Herskowitz Lab)
· Transformation of Yeast - LiAc Method (Lazo Lab)
· Two Hybrid System Transformation (Gietz Lab)
This Protocol is for 2 Hybrid system screens
· Yeast Transformation (Fangman/Brewer lab)
Yeast transformation protocol Using Frozen Competent Cells and Single-stranded DNA as a Carrier
· Quick Yeast Transformation (Gietz Lab)
This Protocol allows for transformation with any Yeast cell source. When just a few transformants are sufficient, such as the transformation of a test plasmid or a GAL4BD fusion plasmid, the following protocol can be used. This procedure is very flexible and can be applied to yeast cells from a number of different sources; however, for best results, use cells from a freshly grown plate.
· Yeast Transformation (D. Amberg)
Electroporation method
· Fission Yeast Transformation (Forsburg Lab)
Includes three methods for transforming S. pombe with recombinant DNA: electroporation, lithium acetate treatment, and protoplast fusion.
· Multiwell Transformation (Davis Lab)
Detailed protocol with LiAcetate PEG transformation method
· Transformation of Yeast (PMCI Research)
· "Lazy Bones" Plasmid Transformation (Dohlman Lab)
A quick method for plasmid transformation into yeast.
· Competent Yeast Preparation (Gietz Lab)
This Protocol allows you to prepare frozen yeast cells that are competent for transformation after thawing
· Yeast Transformation (Gottschling Lab)
Provides LiAc method, "lazy bones" method and electroporation method
· Yeast Transformation (Finley Lab)
LiOAc method
Yeast Transformation with DMSO/LioAc (high efficiency) (Hahn Lab)
Yeast Transformation (Rapid method) (Hahn Lab)
LiOAc method
· Plasmid Rescue from Yeast from Liquid Culture and Clony Rescue (Gottschling Lab)
· Yeast Colony PCR (Gottschling Lab)
For yeast transformant screening
· Direct PCR from Whole Yeast Cells (Namjin Chung)
Zymolyase Method for screening yeast transformants