ImmunohistochemistyFluorescenceProtocol1
MaterialsCytokine-specific Primary Antibodiesunlabeled or biotinylated antigen-affinity purified polyclonal antibodies (R&D Systems ''AF'' or ''BAF'' series) or selected monoclonal antibodies.Secondary Antibodies and Secondary ReagentsBiotinylated donkey anti-goat IgG (Jackson Immuno Research Lab Catalog # 705-066-147)Biotinylated goat anti-mouse IgG1 (Caltag Lab Catalog # M32115)F......閱讀全文
Protocol－for－dsRNA－Synthesis
實驗概要? ? ? ? We routinely produce dsRNA by in vitro transcription of a PCR generated DNA template containing the T7 promoter sequence on both ends
Western－Blotting－Protocol
實驗概要The western blot ?(sometimes called the protein immunoblot) is a widely used analytical ?technique used to detect specific proteins in the given s
Cell－Extraction－Protocol
實驗概要Primary tissues ?are valuable tools for the study of intracellular and extracellular ?markers which characterize disease states. We have developed
Histone－blotting－protocol
實驗概要?Western blot detection of histone proteins.?實驗步驟?The ?following protocol refers to the western blot detection of histone ?proteins derived from p
Yale－Immunofluorescence－Protocol
實驗概要We provide a protocol for fixation, immunostaining, and imaging in 384-well Plates.主要試劑Reagents1.?384-well view plates (Aurora)2.?HUVEC (pooled, L
Immunofluorescence－Microscopy－Protocol
實驗概要Immunofluorescence ?allows the imaging of a specific factor in cells or tissue sections ?through the use of a specific antibody chemically which i
BrdU－Labeling－Protocol
實驗概要The thymidine analog, 5-bromo-2-deoxyuridine (BrdU),is a common reagent used for cell proliferation assays and for the detection of apoptotic
Immunofluorescence－Microscopy－Protocol
實驗概要Immunofluorescence ?allows the imaging of a specific factor in cells or tissue sections ?through the use of a specific antibody chemically which i
Nuclear－Extraction－Protocol
實驗概要The procedure presented below describes a method for extracting nuclear from several cell lines of human origin.主要試劑Hypotonic Buffer Solution20 mM
TAIL－PCR－Protocol
TAIL is a series of reactions that are intended to map where a T-DNA (transfer DNA) has inserted within the genome. The main components of the 3 react
Nucleolar－Isolation－Protocol
We recommend that you first download and read this page as a?PDF file. Using that as your guide, you can then follow the protocol below and view a Qui
Microarray－Hybridization－Protocol
Introduction:One microarray set consists of 7 nylon membranes with 2.5 x 7.5 cm dimension. 2304 genes were spotted onto nylon membranes (Schleicher an
Dot－blot－protocol
實驗概要A ?technique for detecting, analyzing, and identifying proteins, similar ?to the western blot technique but differing in that protein samples are
Basic－ELISA－Protocol
實驗概要? ? ? ? There are many different types of ELISAs, which can detect the presence of protein in serum or supernatent. One of the most common typ
Streamlined－DNA－Extraction－Protocol
This method is derived from a procedure developed by Toby Bradshaw and the Poplar Molecular Genetics Cooperative. We have tested the procedure wit
Direct/Indirect－Staining－Protocol
Use this Protocol for Directly or Indirectly staining cells for Flow Cytometric Analysis1) Dilute cells to 5x10^6 cells/mL2) Aliquot 100uL of cells pe
Xenograft－Tumor－Assay－Protocol
1) Determine the number of cells for injection (ie 5′106 ) to determine the number of plates thatwill require trypsinizing (usually a 100% confluent p
Immunofluorescence－/－Confocal－Microscopy－Protocol
實驗概要Immunofluorescence ?is a technique used for light microscopy with a fluorescence microscope ?and is used primarily on biological samples. This tec
Protocol－for－Construction－of－BAC－Libraries
Protocol for Construction of BAC Libraries??????The bacterial artificial chromosome cloning (BAC) system is emerging as the system of choice for const
ELISA－Protocol－(General－Guidelines)
實驗概要Sandwich ?enzyme-linked immunosorbent assays (ELISAs) involve attachment of a ?capture antibody to a solid phase support. Samples containing known
Tissue－preparation－protocol－for－ChIP
實驗概要This protocol ?describes how chromatin is prepared from tissue, which can subsequently ?be used for chromatin immunoprecipitation (ChIP). It is re
stem－cell－culture－protocol
實驗概要stem cell culture protocol主要試劑cell culture supplies and reagentssEnvironment: cell culture requires a sterile environment, so it needs a separat
IHC－Protocol－for－Fr...
實驗概要The method provides a IHC protocol for free floating brain sections.實驗步驟1.?Coronal 30-40 μm sections cut on a freezing microtome. Sections colle
Native－chromatin－immunoprecipitation－protocol
實驗概要Native chromatin immunoprecipitation to query specific chromatin states of individual genes.?主要試劑10 x TBS 0.1 M Tris-HCl (pH 7.5) 1.5 M NaCl 30 mM
Xenograft－Tumor－Model－Protocol
Preparation of tumor cellsGrow cells in complete medium and exclude any contaminationWhen cells are 70-80% confluent, 3-4 hrs before harvesting, repla
Immunofluorescence－/－Confocal－Microscopy－Protocol
實驗概要Immunofluorescence ?is a technique used for light microscopy with a fluorescence microscope ?and is used primarily on biological samples. This tec
Arabidopsis－RNA－extraction－protocol
1-2 g fresh material, freezer-dried, ground with 0.2g sand (if necessary), and then homogenized with 10ml RNA extraction buffer (see below). Spin
ELISA－Protocol－(General－Guidelines)
實驗概要Sandwich ?enzyme-linked immunosorbent assays (ELISAs) involve attachment of a ?capture antibody to a solid phase support. Samples containing known
ImmunohistochemistyEnzymatic－Protocol
OverviewR&D Systems provides monoclonal, polyclonal and biotinylated antibodies for immunohistochemical use. The following protocol has been developed
Simplified－Arabidopsis－Transformation－Protocol
(Brief version for those who are familiar with the method)Steve Clough and Andrew Bent, University of Illinois at Urbana-Champaign.Our present proto